Anti-WTAP antibody (ab245628)
Key features and details
- Rabbit polyclonal to WTAP
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-WTAP antibody
See all WTAP primary antibodies -
Description
Rabbit polyclonal to WTAP -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Xenopus laevis, Xenopus tropicalis
-
Immunogen
Synthetic peptide within Human WTAP aa 346-396. The exact sequence is proprietary. NP_004897.2
Database link: Q15007 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human testicular seminoma tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245628 was affinity purified using an epitope specific to WTAP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab245628)
Formalin-fixed, paraffin-embedded human testicular seminoma tissue stained for WTAP using ab245628 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
-
Western blot - Anti-WTAP antibody (ab245628)All lanes : Anti-WTAP antibody (ab245628) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 30 secondsLysates prepared in NETN buffer.
-
Immunoprecipitation - Anti-WTAP antibody (ab245628)WTAP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245628 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab245628 at 1 µg/ml.
Lane 1: ab245628 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Lysates prepared in NETN buffer.
