Anti-MMP7 antibody (ab5706)
Key features and details
- Rabbit polyclonal to MMP7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MMP7 antibody
See all MMP7 primary antibodies -
Description
Rabbit polyclonal to MMP7 -
Host species
Rabbit -
Specificity
This antibody specifically recognises MMP7, and does not cross react with MMP1, 2, 3, 8, 9, 10 or 16. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to C-Terminus of MMP7 (Human).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody (ab5706)
Immunohistochemistry analysis of human prostate carcinoma stained with rabbit polyclonal anti-MMP-7 (ab5706).
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Western blot - Anti-MMP7 antibody (ab5706)All lanes : Anti-MMP7 antibody (ab5706)
Lane 1 : Mouse brain lysate
Lane 2 : Human MMP-7 over-expressing Hek293 cell lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody (ab5706)ab5706 (2µg/ml) staining MMP7 in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei within proximal convoluted tubules.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
