Anti-MMP14 antibody - Hinge region (ab38971)
Key features and details
- Rabbit polyclonal to MMP14 - Hinge region
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMP14 antibody - Hinge region
See all MMP14 primary antibodies -
Description
Rabbit polyclonal to MMP14 - Hinge region -
Host species
Rabbit -
Specificity
This antibody binds to MMP14, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9). -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MMP14.
(Peptide available asab41091)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MMP14 antibody - Hinge region (ab38971)All lanes : Anti-MMP14 antibody - Hinge region (ab38971)
Lane 1 : Cell lysates from human dermal fibroblasts neonatal (no treatment)
Lane 2 : Cell lysates from human dermal fibroblasts neonatal (treated with II1beta)
Predicted band size: 66 kDa
Observed band size: 66 kDa
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Western blot - Anti-MMP14 antibody - Hinge region (ab38971) This image is courtesy of an anonymous AbreviewAnti-MMP14 antibody - Hinge region (ab38971) at 1/1000 dilution + HT1080 whole cell lysate at 30 µg
Secondary
HRP-conjugated donkey anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Western blot - Anti-MMP14 antibody - Hinge region (ab38971) This image is a courtesy of an anonymous AbreviewAll lanes : Anti-MMP14 antibody - Hinge region (ab38971) at 1/5000 dilution
Lane 1 : Whole cell lysate prepared from HT1080 cells
Lane 2 : Whole cell lysate prepared from human vascular smooth muscle cells
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : HRP conjugated donkey anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 63,65 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa (possible non-specific binding)
Exposure time: 30 seconds
Blocked using 5% milk for 1 hour at 20°C.
