Recombinant human MMP2 protein (Active) (ab125181)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human MMP2 protein (Active)
See all MMP2 proteins and peptides -
Biological activity
Recombinant Human MMP-2 protein is a pro form. It needs to be activated with p-aminophenylmercuric acetate (APMA).
The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-DpaAR-NH2.
The specific activity is > 1,000 pmoles/min/µg.
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Purity
> 95 % SDS-PAGE.
ab125181 is purified by proprietary chromatographic techniques. -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
APSPIIKFPG DVAPKTDKEL AVQYLNTFYG CPKESCNLFV LKDTLKKMQK FFGLPQTGDL DQNTIETMRK PRCGNPDVAN YNFFPRKPKW DKNQITYRII GYTPDLDPET VDDAFARAFQ VWSDVTPLRF SRIHDGEADI MINFGRWEHG DGYPFDGKDG LLAHAFAPGT GVGGDSHFDD DELWTLGEGQ VVRVKYGNAD GEYCKFPFLF NGKEYNSCTD TGRSDGFLWC STTYNFEKDG KYGFCPHEAL FTMGGNAEGQ PCKFPFRFQG TSYDSCTTEG RTDGYRWCGT TEDYDRDKKY GFCPETAMST VGGNSEGAPC VFPFTFLGNK YESCTSAGRS DGKMWCATTA NYDDDRKWGF CPDQGYSLFL VAAHEFGHAM GLEHSQDPGA LMAPIYTYTK NFRLSQDDIK GIQELYGASP DIDLGTGPTP TLGPVTPEIC KQDIVFDGIA QIRGEIFFFK DRFIWRTVTP RDKPMGPLLV ATFWPELPEK IDAVYEAPQE EKAVFFAGNE YWIYSASTLE RGYPKPLTSL GLPPDVQRVD AAFNWSKNKK TYIFAGDKFW RYNEVKKKMD PGFPKLIADA WNAIPDNLDA VVDLQGGGHS YFFKGAYYLK LENQSLKSVK FGSIKSDWLG C -
Predicted molecular weight
71 kDa including tags -
Amino acids
30 to 660 -
Tags
His tag C-Terminus -
Additional sequence information
Full length mature protein, minus the signal peptide.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Constituents: 0.05% Brij, 0.32% Tris HCl, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human MMP2 protein (Active) (ab125181) This image is courtesy of an Abreview submitted by Mr Pramod MahajanDetection of gelatinolytic activity of ab125181 MMP2 protein.
10 ng of ab125181 in 25 µl of 25 mM Tris-glycine buffer (pH 6.8) was mixed with 2x SDS-sample buffer (without ß-mercaptoethanol) and incubated at 37°C for 10 minutes. Samples were loaded on a 10% acrylamide-Tris-glycine gel containing 0.1% gelatin and electrophoresed using Tris-glycine (pH 8.3) with 0.1% SDS. Electrophoresis was performed at 90V for 2 hours. The gel was washed 3 times in 40-50 ml of 2.5% Triton X-100 for 15 minutes. The gel was immersed in 40-50 ml of 50 mM Tris-glycine (pH 7.5), 100 mM NaCl, 4 mM CaCl2 and 0.02% Triton X-100 and incubated at 37°C for 16 hours. For detection of the gelatinolytic activity, the gel was stained with 0.05% Commassie Blue.
ab125181 produced a prominent gelatinolytic band corresponding to a molecular mass of ~ 68 kDa.
