Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MLX-interacting protein antibody (ab77294)

Key features and details

  • Rabbit polyclonal to MLX-interacting protein
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MLX-interacting protein antibody
    See all MLX-interacting protein primary antibodies

  • Description

    Rabbit polyclonal to MLX-interacting protein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human MLX-interacting protein aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90826)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human heart; Human liver; Human kidney, as well as the following whole cell lysates: HeLa; HepG2; HEK293.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab77294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Relevance

    MLX-interacting protein is a basic helix-loop-helix leucine zipper transcription factor of the Myc/Max/Mad superfamily. This protein binds to DNA as a heterdimer with MLX, resulting in transcription activation. MLX-interacting protein plays a role in transcriptional activation of glycolytic target genes and is involved in glucose-responsive gene regulation.

  • Cellular localization

    Cytoplasmic, Mitochondrial uter membrane and Nuclear. Note: Predominantly cytoplasmic but shuttles between cytoplasm and nucleus when associated with MLX. Also associates with the outer mitochondrial membrane and may shuttle between the outer mitochondrial membrane and the nucleus.

  • Database links

    • Alternative names

      • BHLHE36 antibody
      • Class E basic helix loop helix protein 36 antibody
      • MIR antibody
      • MLXIP antibody
      • MONDOA antibody
      • Transcriptional activator MondoA antibody
      see all

    Images

    • Western blot - Anti-MLX-interacting protein antibody (ab77294)
      Western blot - Anti-MLX-interacting protein antibody (ab77294)
      All lanes : Anti-MLX-interacting protein antibody (ab77294) at 1 µg/ml

      Lane 1 : Human heart tissue lysate - total protein (ab29431)
      Lane 2 : Human liver tissue lysate - total protein (ab29889)
      Lane 3 : Human kidney tissue lysate - total protein (ab30203)
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 101 kDa
      Observed band size: 101 kDa
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 5 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-MLX-interacting protein antibody (ab77294)
      Immunocytochemistry/ Immunofluorescence - Anti-MLX-interacting protein antibody (ab77294)
      ICC/IF image of ab77294 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77294, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

    References (0)

    Publishing research using ab77294? Please let us know so that we can cite the reference in this datasheet.

    ab77294 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-MLX-interacting protein antibody (ab77294)
      Western blot - Anti-MLX-interacting protein antibody (ab77294)
      All lanes : Anti-MLX-interacting protein antibody (ab77294) at 1 µg/ml

      Lane 1 : Human heart tissue lysate - total protein (ab29431)
      Lane 2 : Human liver tissue lysate - total protein (ab29889)
      Lane 3 : Human kidney tissue lysate - total protein (ab30203)
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 101 kDa
      Observed band size: 101 kDa
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 5 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-MLX-interacting protein antibody (ab77294)
      Immunocytochemistry/ Immunofluorescence - Anti-MLX-interacting protein antibody (ab77294)
      ICC/IF image of ab77294 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77294, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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