Anti-MICA + MICB antibody [EPR22071-91] - BSA and Azide free (ab254196)
![Anti-MICA + MICB antibody [EPR22071-91] - BSA and Azide free (ab254196)](https://www.abcam.com/ps/products/254/ab254196/Images/ab254196-1-222098IP1.jpg "Anti-MICA + MICB antibody [EPR22071-91] - BSA and Azide free (ab254196)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22071-91] to MICA + MICB - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-MICA + MICB antibody [EPR22071-91] - BSA and Azide free -
Description
Rabbit monoclonal [EPR22071-91] to MICA + MICB - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: K562 whole cell lysate.
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General notes
Ab254196 is the carrier-free version of ab222098. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254196 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22071-91 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-MICA + MICB antibody [EPR22071-91] - BSA and Azide free (ab254196)
MICA + MICB were immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab222098 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222098 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: K562 whole cell lysate 10 μg (Input).
Lane 2: ab222098 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222098 in K562 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222098).
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![Anti-MICA + MICB antibody [EPR22071-91] - BSA and Azide free (ab254196)](https://www.abcam.com/ps/products/254/ab254196/Images/ab254196-2-benefits-of-recombinant-antibodies.png)
Anti-MICA + MICB antibody [EPR22071-91] - BSA and Azide free (ab254196)