Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-METTL16 antibody [EPR23459-39] (ab252420)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23459-39] to METTL16
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-METTL16 antibody [EPR23459-39]
    See all METTL16 primary antibodies

  • Description

    Rabbit monoclonal [EPR23459-39] to METTL16

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7, RAW 264.7, HeLa and MCF7, RAW 264.7 lysates. IP: HeLa cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23459-39

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-METTL16 antibody [EPR23459-39] (ab252420)
    Western blot - Anti-METTL16 antibody [EPR23459-39] (ab252420)
    All lanes : Anti-METTL16 antibody [EPR23459-39] (ab252420) at 1/1000 dilution

    Lanes 1 & 4 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lanes 2 & 5 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lanes 3 & 6 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 64 kDa
    Observed band size: 63 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates were made freshly and used in WB test immediately to minimize protein degradation (lanes 1-3). Lanes 4-6 are the lysates from same cell lines but have experienced freeze-thaw cycle.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29051200, 31940410). 

    Exposure time: 3 minutes

  • Immunoprecipitation - Anti-METTL16 antibody [EPR23459-39] (ab252420)
    Immunoprecipitation - Anti-METTL16 antibody [EPR23459-39] (ab252420)

    METTL16 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab252420 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252420 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab252420 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252420 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 69 seconds

    The band below 50 kDa is caused by degradation as demonstrated by WB data.

  • Anti-METTL16 antibody [EPR23459-39] (ab252420)
    Anti-METTL16 antibody [EPR23459-39] (ab252420)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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