Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)
![Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)](https://www.abcam.com/ps/products/216/ab216330/Images/ab216330-4-216330WB1.jpg "Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22436-24] to Met (c-Met)
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Mouse
Overview
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Product name
Anti-Met (c-Met) antibody [EPR22436-24]
See all Met (c-Met) primary antibodies -
Description
Rabbit monoclonal [EPR22436-24] to Met (c-Met) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF MouseIP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: B16-F10 whole cell lysate. ICC/IF: B16-F10 cells. Flow: B16-F10 cells. IP: B16-F10 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22436-24 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)All lanes : Anti-Met (c-Met) antibody [EPR22436-24] (ab216330) at 1/1000 dilution
Lane 1 : B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 155 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 26 seconds.
Negative control: NIH/3T3 (PMID: 21496277; PMID: 8197126; PMID 9888438)
The molecular weight observed is consistent with what has been described in the literature (PMID: 8710887).
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![Immunoprecipitation - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)](https://www.abcam.com/ps/products/216/ab216330/Images/ab216330-3-216330IP1.jpg)
Immunoprecipitation - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)Met (c-Met) was immunoprecipitated from 0.35mg of B16-F10 (Mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate using ab216330 at 1/30 dilution. WB was performed on the immunoprecipitate using ab216330 at 1/1000 dilution, followed by the VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution.
Lane 1: B16-F10 whole cell lysate 10µg (input).
Lane 2: ab216330 IP in B16-F10 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216330 in B16-F10 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds. -
![Flow Cytometry - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)](https://www.abcam.com/ps/products/216/ab216330/Images/ab216330-2-216330FC1.jpg)
Flow Cytometry - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast, Left) / B16-F10 (Mouse melanoma mixture of spindle-shaped and epithelial-like cells, Right) labeling Met (c-Met) with ab216330 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody, Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.
Negative control: NIH/3T3(PMID: 21496277, 8197126, 9888438).
Gated on viable cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)](https://www.abcam.com/ps/products/216/ab216330/Images/ab216330-1-216330ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) and NIH/3T3 (mouse embryonic fibroblast) cells labelling Met (c-Met) with ab216330 with ab216330 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in B16-F10 cell line. Tubulin was counterstained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. The nuclear counterstain was DAPI (Blue).
Negative control: NIH/3T3 (PMID: 21496277, 8197126, 9888438)
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![Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)](https://www.abcam.com/ps/products/216/ab216330/Images/ab216330-5-benefits-of-recombinant-antibodies.png)
Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)
