Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22436-24] to Met (c-Met)
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Met (c-Met) antibody [EPR22436-24]
    See all Met (c-Met) primary antibodies

  • Description

    Rabbit monoclonal [EPR22436-24] to Met (c-Met)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: B16-F10 whole cell lysate. ICC/IF: B16-F10 cells. Flow: B16-F10 cells. IP: B16-F10 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)
    Western blot - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)
    All lanes : Anti-Met (c-Met) antibody [EPR22436-24] (ab216330) at 1/1000 dilution

    Lane 1 : B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 155 kDa
    Observed band size: 175 kDa
    why is the actual band size different from the predicted?



    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: 26 seconds.

    Negative control: NIH/3T3 (PMID: 21496277; PMID: 8197126; PMID 9888438)

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8710887).

  • Immunoprecipitation - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)
    Immunoprecipitation - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

    Met (c-Met) was immunoprecipitated from 0.35mg of B16-F10 (Mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate using ab216330 at 1/30 dilution. WB was performed on the immunoprecipitate using ab216330 at 1/1000 dilution, followed by the VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution.

    Lane 1: B16-F10 whole cell lysate 10µg (input).
    Lane 2: ab216330 IP in B16-F10 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216330 in B16-F10 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

     

  • Flow Cytometry - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)
    Flow Cytometry - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

    Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast, Left) / B16-F10 (Mouse melanoma mixture of spindle-shaped and epithelial-like cells, Right) labeling Met (c-Met) with ab216330 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody, Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

    Negative control: NIH/3T3(PMID: 21496277, 8197126, 9888438).

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)
    Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) and NIH/3T3 (mouse embryonic fibroblast) cells labelling Met (c-Met) with ab216330 with ab216330 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in B16-F10 cell line.  Tubulin was counterstained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

    Negative control: NIH/3T3 (PMID: 21496277, 8197126, 9888438)

  • Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)
    Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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