Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

250 µl 321 638 ₸

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Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)

Key features and details

Mouse monoclonal [HMB45 + M2-7C10 + M2-9E3 + T311] to Melanoma
Suitable for: Flow Cyt, IHC-P
Reacts with: Human
Isotype: IgG

Product name

Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311]
See all Melanoma primary antibodies
Description

Mouse monoclonal [HMB45 + M2-7C10 + M2-9E3 + T311] to Melanoma
Host species

Mouse
Specificity

The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. MART-1 recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45.
Tested applications

Suitable for: Flow Cyt, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein Tyrosinase - Recombinant tyrosinase protein
Positive control
- Metastatic melanoma in lymph node.
General notes

Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

The combination of HMB45, MART-1 (M2-7C10 + M2-9E3) and Tyrosinase (T311) (SwissProt: P14679 Human, Omim: 606933 Human, Entrez Gene: 22173 Mouse, Entrez Gene: 308800 Rat) make this quadruple antibody cocktail a first-order pan melanoma screener, and may prove to be a valuable marker for melanoma metastasis in sentinel lymph nodes (see reference 3.).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.1% Sodium azide
Constituents: PBS, Tissue culture supernatant

Proprietary preservative that is not sodium azide or thimerosal, protein carrier.
Concentration information loading...
Primary antibody notes

The combination of HMB45, MART-1 (DT101 + BC199) and Tyrosinase (T311) make this quadruple antibody cocktail a first-order pan melanoma screener, and may prove to be a valuable marker for melanoma metastasis in sentinel lymph nodes (see reference 3.).
Clonality

Monoclonal
Clone number

HMB45 + M2-7C10 + M2-9E3 + T311
Myeloma

unknown
Isotype

IgG
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)

IHC image of ab733 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab733, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)

ab733 staining Melanoma by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Flow Cytometry - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)

Overlay histogram showing MALME 3M cells stained with ab733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab733, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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