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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)

Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3338] to MEK1 (phospho S298) - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free
    See all MEK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3338] to MEK1 (phospho S298) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    ab96379 detects MEK1 phosphorylated at threonine 298.

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HeLa cells; ICC: HeLa cells; IHC-P: Human ovarian carcinoma tissue; WB; Rat and mouse skeletal muscle, HeLa cells.
  • General notes

    Ab214445 is the carrier-free version of ab96379. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab214445 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3338
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Images

  • Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379) at 1/1000 dilution (Purified)

    Lane 1 : Rat skeletal muscle lysate
    Lane 2 : Rat skeletal muscle lysate, the membrane treated with phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa

  • Flow Cytometry - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    Flow Cytometry - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    This data was developed using ab96379, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MEK1 with Purified ab96379 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379) at 1/1000 dilution (Purified)

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Mouse skeletal muscle lysate, the membrane treated with phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa

  • Immunocytochemistry - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    Immunocytochemistry - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    This data was developed using ab96379, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with lambda phosphatase cells labeling MEK1 with Purified ab96379 at 1/100 dilution (9.53 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100 ng/ml nocoladaze for 18 hours whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml nocoladaze for 18 hours, then the membrane treated with phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    This data was developed using ab96379, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling MEK1 with Purified ab96379 at 1/50 dilution (19.06 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)
    Anti-MEK1 (phospho S298) antibody [EPR3338] - BSA and Azide free (ab214445)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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