Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1452(N)] to MEF2C - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free
    See all MEF2C primary antibodies

  • Description

    Rabbit monoclonal [EPR1452(N)] to MEF2C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human MEF2C aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q06413

  • General notes

    Ab251079 is the carrier-free version of ab191428. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251079 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)

    This data was developed using ab191428, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab:ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    Western blot - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    All lanes : Anti-MEF2C antibody [EPR1452(N)] (ab191428) at 1/1000 dilution

    Lane 1 : Saos-2 cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab191428, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    Western blot - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    Anti-MEF2C antibody [EPR1452(N)] (ab191428) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa
    Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab191428, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    Western blot - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    Anti-MEF2C antibody [EPR1452(N)] (ab191428) at 1/1000 dilution + Rat brain tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab191428, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    This data was developed using ab191428, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab: ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    This data was developed using ab191428, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab: ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)
    Anti-MEF2C antibody [EPR1452(N)] - BSA and Azide free (ab251079)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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