Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13382-9] to IQGAP2
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-IQGAP2 antibody [EPR13382-9]
See all IQGAP2 primary antibodies
Description

Rabbit monoclonal [EPR13382-9] to IQGAP2
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2 and Ramos cell lysate, Rat liver and Mouse testis tissue lysate. IHC-P: Human prostatic hyperplasia, colonic carcinoma and Mouse testis tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13382-9
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling IQGAP2 with Purified ab187153 at 1:100 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

All lanes : Anti-IQGAP2 antibody [EPR13382-9] (ab187153) at 0.1 µg/ml (purified)

Lane 1 : Rat liver lysates
Lane 2 : Mouse testis lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 181 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Anti-IQGAP2 antibody [EPR13382-9] (ab187153) at 0.01 µg/ml (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 181 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Immunohistochemical analysis of paraffin-embedded Mouse testis labeling IQGAP2 with unpurified ab187153 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma labeling IQGAP2 with unpurified ab187153 at 1/100 dilution.
Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

All lanes : Anti-IQGAP2 antibody [EPR13382-9] (ab187153) at 1/1000 dilution (Unpurified)

Lane 1 : HepG2 cell lysate
Lane 2 : Ramos cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 181 kDa
Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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