Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4B4] to MeCP2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Rat

Overview

  • Product name

    Anti-MeCP2 antibody [4B4] - BSA and Azide free
    See all MeCP2 primary antibodies

  • Description

    Mouse monoclonal [4B4] to MeCP2 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat brain nuclear fraction lysate. IHC-P: Rat cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255834 is the carrier-free version of ab252840. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255834 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)
    Western blot - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)
    All lanes : Anti-MeCP2 antibody [4B4] (ab252840) at 1/1000 dilution

    Lane 1 : Rat brain cytoplasmic fraction
    Lane 2 : Rat brain nuclear fraction

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 52 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.   

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252840).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MeCP2 with ab252840 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat cerebrum. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252840).

  • Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)
    Anti-MeCP2 antibody [4B4] - BSA and Azide free (ab255834)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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