Anti-MCM6 antibody (ab190948)
Key features and details
- Rabbit polyclonal to MCM6
- Suitable for: Flow Cyt, IHC-P, IHC-Fr, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MCM6 antibody
See all MCM6 primary antibodies -
Description
Rabbit polyclonal to MCM6 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, IHC-Fr, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to Human MCM6 aa 550-650 (internal sequence).
Database link: Q14566 -
Positive control
- Flow Cyt: SiHa cells ICC: human A431 cells; HeLa cells WB: mouse small intestine and spleen tissue lysate; u87, COLO320, HeLa, MCF-7, Jurkat cells IHC-P: rat intestine tissue; rat brain tissue; human intestinal cancer IHC-F: human placenta tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-MCM6 antibody (ab190948)
MCM6 was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190948 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Western blot - Anti-MCM6 antibody (ab190948)All lanes : Anti-MCM6 antibody (ab190948) at 0.5 µg/ml
Lane 1 : U87 cell lysate
Lane 2 : COLO320 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : Jurkat cell lysate
Predicted band size: 93 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab190948)Immunohistochemistry of paraffin-embedded Human Intestinal Cancer Tissue labelling MCM6 with ab190948 at 0.5 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab190948)Immunohistochemistry of paraffin-embedded Rat Brain Tissue labelling MCM6 with ab190948 at 0.5 µg/ml.
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Immunocytochemistry - Anti-MCM6 antibody (ab190948)Immunocytochemical analysis of HeLa cells, staining MCM6 with ab190948 at 1 µg/ml.
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Immunohistochemistry (Frozen sections) - Anti-MCM6 antibody (ab190948)Immunohistochemistry of Frozen section of Human Placenta Tissue labelling MCM6 with ab190948 at 0.5 µg/ml.
