Anti-MCM6 antibody (ab184147)
Key features and details
- Rabbit polyclonal to MCM6
- Suitable for: WB, ICC/IF, IHC-P, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCM6 antibody
See all MCM6 primary antibodies -
Description
Rabbit polyclonal to MCM6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, ChIPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey
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Immunogen
Recombinant fragment within Human MCM6 aa 682-821 (C terminal). The exact sequence is proprietary.
Database link: Q14566 -
Positive control
- 293T, A431, HeLa or HepG2 lysate, Human ovarian carcinoma or gastric carcinoma tissue, U2OS cells, HeLa chromatin extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM6 antibody (ab184147)All lanes : Anti-MCM6 antibody (ab184147) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : A431 lysate
Lane 3 : HeLa lysate
Lane 4 : HepG2 lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 93 kDa7.5 % SDS-PAGE
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ChIP - Anti-MCM6 antibody (ab184147)Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either normal rabbit IgG (1) or ab184147 (2). The precipitated DNA was detected by PCR with primer set targeting to Myc promoter (1 and 2).
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Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody (ab184147)Immunofluorescence analysis of 4% paraformaldehyde fixed U2OS cells, labeling MCM6 with ab184147 at a 1/500 dilution (left image), and costained with Hoescht 33342 (right image).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab184147)Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab184147)Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.
