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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing

Anti-MBNL1 antibody (ab77017)

Anti-MBNL1 antibody (ab77017)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal to MBNL1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-MBNL1 antibody
    See all MBNL1 primary antibodies
  • Description

    Mouse monoclonal to MBNL1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human MBNL1. (AAH50535)

  • Positive control

    • HeLa whole cell lysate (ab150035); recombinant full length human MBNL1 (the immunogen).
  • General notes

     This product was previously labelled as Muscleblind-like 1

    This product was changed from ascites to tissue culture supernatant on 30th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Images

  • Western blot - Anti-MBNL1 antibody (ab77017)
    Western blot - Anti-MBNL1 antibody (ab77017)
    Anti-MBNL1 antibody (ab77017) at 5 µg/ml + recombinant tagged full length human MBNL1 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size: 41 kDa
    Observed band size: 64 kDa
    why is the actual band size different from the predicted?



    Molecular weight of the tag alone is 26 kDa.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-MBNL1 antibody (ab77017)
    Western blot - Anti-MBNL1 antibody (ab77017)
    Anti-MBNL1 antibody (ab77017) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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