Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
![Anti-MBD2 + MBD3 antibody [106B691] (ab45027)](https://www.abcam.com/ps/products/45/ab45027/Images/ab45027-57153-anti-mbd2-mbd3-antibody-106b691-western-blot.jpg "Anti-MBD2 + MBD3 antibody [106B691] (ab45027)")
Key features and details
- Mouse monoclonal [106B691] to MBD2 + MBD3
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MBD2 + MBD3 antibody [106B691] -
Description
Mouse monoclonal [106B691] to MBD2 + MBD3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide:
CKAFMVTDEDIRKQEE
, corresponding to amino acids 215-230 of Human MBD3 -
Positive control
- HeLa cells (nuclear fraction).
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General notes
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. -
Clonality
Monoclonal -
Clone number
106B691 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)All lanes : Anti-MBD2 + MBD3 antibody [106B691] (ab45027) at 2 µg/ml
Lane 1 : HeLa cell lysate (nuclear fraction) without immunising peptide
Lane 2 : HeLa cell lysate (nuclear fraction) with immunising peptide
Predicted band size: 33, 45 kDa
Observed band size: 33,45 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
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![Flow Cytometry - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)](https://www.abcam.com/ps/products/45/ab45027/Images/ab45027-57151-anti-mbd2-mbd3-antibody-106b691-flow-cytometry.jpg)
Flow Cytometry - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)Overlay histogram showing HeLa cells stained with ab45027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45027, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
