Anti-MBD4/MED1 antibody (ab227625)
Key features and details
- Rabbit polyclonal to MBD4/MED1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MBD4/MED1 antibody
See all MBD4/MED1 primary antibodies -
Description
Rabbit polyclonal to MBD4/MED1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human MBD4/MED1 (internal sequence). The exact sequence is proprietary.
Database link: O95243 -
Positive control
- WB: MBD4/MED1 transfected HEK-293T whole cell extract. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as MBD4
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MBD4/MED1 antibody (ab227625)All lanes : Anti-MBD4/MED1 antibody (ab227625) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : MBD4/MED1 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 66 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-MBD4/MED1 antibody (ab227625)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MBD4/MED1 (green) using ab227625 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
