Anti-MBD1 antibody [EPR3564] - BSA and Azide free (ab247676)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3564] to MBD1 - BSA and Azide free
- Suitable for: ICC, IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MBD1 antibody [EPR3564] - BSA and Azide free
See all MBD1 primary antibodies -
Description
Rabbit monoclonal [EPR3564] to MBD1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A431, 293T and Jurkat whole cell lysate (ab7899). IHC-P: Human tonsil tissue. ICC: MCF7 cells.
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General notes
ab247676 is the carrier-free version of ab108510 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247676 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3564 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MBD1 antibody [EPR3564] (ab108510) at 1/500 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : MBD1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 70-80 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab108510).
Lanes 1 - 4: Merged signal (red and green). Green - ab108510 observed at 70-80 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108510 was shown to react with MBD1 in wild-type HAP1 cells in western blot with loss of signal observed in MBD1 knockout sample. Wild-type and MBD1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108510 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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This data was developed using ab108510, the same antibody clone in a different buffer formulation.ab108510, at 1/100 dilution, staining MBD1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-MBD1 antibody [EPR3564] (ab108510) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 67 kDaThis data was developed using ab108510, the same antibody clone in a different buffer formulation.
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