Anti-MAP3K1 (phospho T1400) antibody (ab138662)
Key features and details
- Rabbit polyclonal to MAP3K1 (phospho T1400)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP3K1 (phospho T1400) antibody
See all MAP3K1 primary antibodies -
Description
Rabbit polyclonal to MAP3K1 (phospho T1400) -
Host species
Rabbit -
Specificity
ab138662 detects endogenous levels of MAP3K1 only when phosphorylated at T1400 in Human samples or at T1381 in Mouse samples.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MAP3K1 (internal sequence). Immunogen derived a synthesized phospho-peptide from human MAP3K1 around the phosphorylation site of Thr1400.
Database link: Q13233 -
General notes
This product was previously labelled as MEKK1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab138662 was purified from Rabbit antiserum. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MAP3K1 (phospho T1400) antibody (ab138662)Anti-MAP3K1 (phospho T1400) antibody (ab138662) at 1/500 dilution + extracts from Jurkat cells at 30 µg
Predicted band size: 164 kDa
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Western blot - Anti-MAP3K1 (phospho T1400) antibody (ab138662) This image is courtesy of an anonymous AbreviewAll lanes : Anti-MAP3K1 (phospho T1400) antibody (ab138662) at 1/250 dilution
Lane 1 : Mouse B16F10 whole cell lysate - untreated
Lane 2 : Mouse B16F10 whole cell lysate - treated with 5µM Osteoponin for 10 minutes
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 164 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
