Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17581] to MAGP2 - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra), IP, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-MAGP2 antibody [EPR17581] - BSA and Azide free
    See all MAGP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17581] to MAGP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), IP, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human bladder lysate; HeLa cell lysate; Rat kidney and spleen lysates. IHC-P: Human colon and breast carcinoma tissues. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.

  • General notes

    ab240367 is the carrier-free version of ab203828.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAGP2 with ab203828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203828).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)
    Flow Cytometry - Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling MAGP2 with ab203828 at 1/750 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203828).

  • Immunoprecipitation - Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)
    Immunoprecipitation - Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)

    MAGP2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab203828 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab203828 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab203828 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203828 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203828).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MAGP2 with ab203828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203828).

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)
    Anti-MAGP2 antibody [EPR17581] - BSA and Azide free (ab240367)

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