Anti-Elastin antibody [10B8] (ab77804)
![Anti-Elastin antibody [10B8] (ab77804)](https://www.abcam.com/ps/products/77/ab77804/Images/Elastin-Primary-antibodies-ab77804-1.jpg "Anti-Elastin antibody [10B8] (ab77804)")
Key features and details
- Mouse monoclonal [10B8] to Elastin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Elastin antibody [10B8]
See all Elastin primary antibodies -
Description
Mouse monoclonal [10B8] to Elastin -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Goat, Chicken, Cow, Dog
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Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human skin tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
10B8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [10B8] (ab77804)
IHC image of ab77804 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77804 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [10B8] (ab77804)](https://www.abcam.com/ps/products/77/ab77804/Images/Elastin-Primary-antibodies-ab77804-10.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [10B8] (ab77804) This image is courtesy of an Abreview submitted by Carl Hobbsab77804 staining dog lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes, followed by staining with ab77804 at 1/100 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.
