Anti-LXR beta/NER antibody (ab28479)
Key features and details
- Rabbit polyclonal to LXR beta/NER
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LXR beta/NER antibody
See all LXR beta/NER primary antibodies -
Description
Rabbit polyclonal to LXR beta/NER -
Host species
Rabbit -
Specificity
ab28479 recognises LXR beta/NER. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat LXR beta/NER aa 113-126.
Sequence:RYACRGSG TCQMDA
(Peptide available asab39817) -
Positive control
- Mouse and rat liver
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LXR beta/NER antibody (ab28479)All lanes : Anti-LXR beta/NER antibody (ab28479) at 1/1000 dilution
Lane 1 : Rat liver cell lysate
Lane 2 : Mouse liver cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit
Predicted band size: 51 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-LXR beta/NER antibody (ab28479)ab28479 labelling LXR beta/NER (green) in the nuclear envelope of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-LXR beta/NER antibody (ab28479)ab28479 staining LXR beta/NER in C2C12 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/2000). Nuclei stained blue and F-actin stained red. Panel d shows the merged image
