All lanes : Anti-LXR beta/NER antibody (ab28479) at 1/1000 dilution

Lane 1 : Rat liver cell lysate
Lane 2 : Mouse liver cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
All lanes : HRP-conjugated anti-rabbit

Predicted band size: 51 kDa

ab28479 labelling LXR beta/NER (green) in the nuclear envelope of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

ab28479 staining LXR beta/NER in C2C12 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/2000). Nuclei stained blue and F-actin stained red. Panel d shows the merged image