Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10690] to LSM5 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LSM5 antibody [EPR10690] - BSA and Azide free
    See all LSM5 primary antibodies

  • Description

    Rabbit monoclonal [EPR10690] to LSM5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250769 is the carrier-free version of ab184568.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    Flow Cytometry - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    This data was developed using ab184568, the same antibody clone in a different buffer formulation.Flow cytometry analysis of T47-D cells using ab184568 at a 1/110 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
  • Immunocytochemistry - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    Immunocytochemistry - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    This data was developed using ab184568, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of 4% paraformaldehyde fixed T47-D cells labeling LSM5 with ab184568 at a 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor®555) secondary used at a 1/200 diution. Conterstained with Dapi (right image).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    This data was developed using ab184568, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling LSM5 with ab184568 a 1/100 dilution. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    This data was developed using ab184568, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human astrocytoma tissue labeling LSM5 with ab184568 at a 1/100 dilution. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    Western blot - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    Anti-LSM5 antibody [EPR10690] (ab184568) at 1/10000 dilution + T47-D lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa



    This data was developed using ab184568, the same antibody clone in a different buffer formulation.

     

  • Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
    Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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