Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] (ab270606)
![Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] (ab270606)](https://www.abcam.com/ps/products/270/ab270606/Images/ab270606-397800-anti-lrrk2-phospho-t1357-antibody-mjfr2952-western-blot-flaglrrk2.jpg "Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] (ab270606)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R29-52] to LRRK2 (phospho T1357)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52]
See all LRRK2 primary antibodies -
Description
Rabbit monoclonal [MJF-R29-52] to LRRK2 (phospho T1357) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Immunoprecipitates of overexpressed FLAG-LRRK2 G2019S in HEK293 cells, subjected to an in vitro kinase reaction prior to SDS-PAGE analysis.
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General notes
This antibody was developed with support from The Michael J. Fox Foundation.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R29-52 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] (ab270606)All lanes : Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] (ab270606) at 1/1000 dilution
Lane 1 : Immunoprecipitates of overexpressed FLAG-LRRK2 G2019S in HEK293 cells, subjected to an in vitro kinase reaction prior to SDS-PAGE analysis
Lane 2 : Immunoprecipitates of overexpressed FLAG-LRRK2 D2017A in HEK293 cells, subjected to an in vitro kinase reaction prior to SDS-PAGE analysis
Lysates/proteins at 10 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 286 kDa
Observed band size: 288 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The G2019S leucine-rich repeat kinase 2 (LRRK2) mutation is the most common cause of genetic Parkinson's disease (PD). Transfection of G2019S mutant can lead to the phosphorylation of LRRK2 at Thr1357.
The immunoprecipitates were kindly provided by Dr. Dario Alessi.
Exposure time: 3 minutes.
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![Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] (ab270606)](https://www.abcam.com/ps/products/270/ab270606/Images/ab270606-3-benefits-of-recombinant-antibodies.png)
Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] (ab270606)