Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LPCAT1 antibody [EPR19882] (ab214034)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19882] to LPCAT1
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-LPCAT1 antibody [EPR19882]
    See all LPCAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19882] to LPCAT1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain, testis, fetal spleen, fetal kidney and prostate cancer lysates; A431, A549 and PC-3 whole cell lysates. IHC-P: Human lung, liver cancer and lung cancer tissues. ICC/IF: A431 and A549 cells. Flow Cyt: A549 and A431 cells. IP: A549 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19882

  • Isotype

    IgG

Images

  • Western blot - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Western blot - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Lanes 1-6 : Anti-LPCAT1 antibody [EPR19882] (ab214034) at 1/2000 dilution
    Lanes 7-8 : Anti-LPCAT1 antibody [EPR19882] (ab214034) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human testis lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 5 : Human fetal spleen lysate
    Lane 6 : Human fetal kidney lysate
    Lane 7 : Human prostate cancer lysate
    Lane 8 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4/8: 4 seconds; Lane 5/6: 8 seconds; Lane 7: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT1 antibody [EPR19882] (ab214034)

    Immunohistochemical analysis of paraffin-embedded human lung tissue labeling LPCAT1 with ab214034 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Cytoplasmic or granular staining on the type two alveolar epithelial cells of human lung is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT1 antibody [EPR19882] (ab214034)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling LPCAT1 with ab214034 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Cytoplasmic or granular staining on the liver cancer cells which contain abundant lipid drops is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT1 antibody [EPR19882] (ab214034)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling LPCAT1 with ab214034 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Cytoplasmic or granular staining on the lung cancer cells is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Immunocytochemistry/ Immunofluorescence - Anti-LPCAT1 antibody [EPR19882] (ab214034)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on A431 cells. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Immunocytochemistry/ Immunofluorescence - Anti-LPCAT1 antibody [EPR19882] (ab214034)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on A549 cells. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow Cytometry - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Flow Cytometry - Anti-LPCAT1 antibody [EPR19882] (ab214034)

    Flow cytometric analysis of 4% paraformaldehyde-fixed A549 (Human lung carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/50 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Flow Cytometry - Anti-LPCAT1 antibody [EPR19882] (ab214034)

    Flow cytometric analysis of 4% paraformaldehyde-fixed A431 (Human epidermoid carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Immunoprecipitation - Anti-LPCAT1 antibody [EPR19882] (ab214034)

    LPCAT1 was immunoprecipitated from 0.35 mg of A549 (Human lung carcinoma cell line) whole cell lysate with ab214034 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab214034 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: A549 whole cell lysate 10µg (Input).

    Lane 2: ab214034 IP in A549 whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab214034 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-LPCAT1 antibody [EPR19882] (ab214034)
    Anti-LPCAT1 antibody [EPR19882] (ab214034)

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