Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)
![Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)](https://www.abcam.com/ps/products/247/ab247530/Images/ab247530-3-anti-lmo2-antibody-ep3257-bsa-and-azide-free-immunoprecipitation.jpg "Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3257] to LMO2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LMO2 antibody [EP3257] - BSA and Azide free
See all LMO2 primary antibodies -
Description
Rabbit monoclonal [EP3257] to LMO2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247530 is the carrier-free version of ab91652. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247530 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3257 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)This data was developed using ab91652, the same antibody clone in a different buffer formulation.ab91652 (purified) at 1:30 dilution (2µg) immunoprecipitating LMO2 in Raji whole cell lysate.
Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab91652 & Raji whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab91652 in Raji whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)](https://www.abcam.com/ps/products/247/ab247530/Images/ab247530-405671-anti-lmo2-antibody-ep3257-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)All lanes : Anti-LMO2 antibody [EP3257] (ab91652) at 0.3 µg/ml (purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Mouse E14 embryo tissue lysate
Lane 4 : Rat E14 embryo tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDaThis data was developed using ab91652, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)](https://www.abcam.com/ps/products/247/ab247530/Images/ab247530-397983-anti-lmo2-antibody-ep3257-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)All lanes : Anti-LMO2 antibody [EP3257] (ab91652) at 1/1000 dilution (Unpurified)
Lane 1 : Raji cell lysate
Lane 2 : Fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDaThis data was developed using ab91652, the same antibody clone in a different buffer formulation.
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![Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)](https://www.abcam.com/ps/products/247/ab247530/Images/ab247530-4-benefits-of-recombinant-antibodies.png)
Anti-LMO2 antibody [EP3257] - BSA and Azide free (ab247530)