Anti-LMAN1 antibody [OTI1B8] (ab118407)
![Anti-LMAN1 antibody [OTI1B8] (ab118407)](https://www.abcam.com/ps/products/118/ab118407/Images/ab118407-412977-anti-lman1-antibody-oti1b8-western-blot-hek293t-004104-wt-hek293t-lman1-knockout-hela-daudi.jpg "Anti-LMAN1 antibody [OTI1B8] (ab118407)")
Key features and details
- Mouse monoclonal [OTI1B8] to LMAN1
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Dog, Human, African green monkey
- Isotype: IgG1
Overview
-
Product name
Anti-LMAN1 antibody [OTI1B8]
See all LMAN1 primary antibodies -
Description
Mouse monoclonal [OTI1B8] to LMAN1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Recombinant full length protein corresponding to Human LMAN1. Produced in HEK-293T cells (NP_005561).
Database link: P49257 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY LMAN1 cDNA; HEK-293T, Daudi, A549, COS-7, HeLa, HepG2, HT-29, Jurkat, MCF7, MDCK and PC-12 cell extracts.
-
General notes
Clone OTI1B8 (formerly 1B8).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography.. -
Clonality
Monoclonal -
Clone number
OTI1B8 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)All lanes : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : LMAN1 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab118407 observed at 55 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab118407 was shown to react with LMAN1 in wild-type HEK-293T cells in western blot with loss of signal observed in LMAN1 knockout cell line ab266248 (LMAN1 knockout cell lysate ab257505). Wild-type and LMAN1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab118407 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 200 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)](https://www.abcam.com/ps/products/118/ab118407/Images/LMAN1-Primary-antibodies-ab118407-1.jpg)
Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)All lanes : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY LMAN1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 58 kDa
-
![Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)](https://www.abcam.com/ps/products/118/ab118407/Images/LMAN1-Primary-antibodies-ab118407-2.jpg)
Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)All lanes : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT29 (human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa
-
![Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)](https://www.abcam.com/ps/products/118/ab118407/Images/ab118407-314448-ab118407WB3.jpg)
Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)Lanes 1-2 : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/1000 dilution
Lanes 3-4 : anti-HSP90AB1
Lanes 1 & 3 : Wild-type HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lanes 2 & 4 : LMAN1-knockout HEK-293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 58 kDa
