Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LMAN1 antibody [EPR6980] (ab126720)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6980] to LMAN1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-LMAN1 antibody [EPR6980]
    See all LMAN1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6980] to LMAN1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues in Human LMAN1 (P49257).

  • Positive control

    • WB: HEK-293T, HeLa, NCI-H460 and JAR cell lysates. IHC-P: Papillary adenocarcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
    Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
    All lanes : Anti-LMAN1 antibody [EPR6980] (ab126720) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : LMAN1 knockout HEK-293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab126720 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab126720 Anti-LMAN1 antibody [EPR6980] was shown to specifically react with Protein ERGIC-53 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266248 (knockout cell lysate ab257505) was used. Wild-type and Protein ERGIC-53 knockout samples were subjected to SDS-PAGE. ab126720 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°CC at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
    Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
    All lanes : Anti-LMAN1 antibody [EPR6980] (ab126720) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : NCI-H460 cell lysates
    Lane 3 : JAR cell lysates

    Predicted band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6980] (ab126720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6980] (ab126720)

    ab126720, at 1/50 dilution, stains LMAN1 in paraffin-embedded papillary adenocarcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-LMAN1 antibody [EPR6980] (ab126720)
    OI-RD Scanning - Anti-LMAN1 antibody [EPR6980] (ab126720)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-LMAN1 antibody [EPR6980] (ab126720)
    Anti-LMAN1 antibody [EPR6980] (ab126720)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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