Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21844-108] to LINE-1 ORF1p
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-LINE-1 ORF1p antibody [EPR21844-108]
    See all LINE-1 ORF1p primary antibodies

  • Description

    Rabbit monoclonal [EPR21844-108] to LINE-1 ORF1p

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: F9 whole cell lysate and mouse testis lysate. IHC-P: Mouse testis tissue. ICC/IF: F9 cells. Flow Cyt: F9 cells. IP: F9 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21844-108

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling LINE-1 ORF1p with ab216324 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on spermatogonia of mouse testis (PMID: 24607009) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    Western blot - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    All lanes : Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) at 1/1000 dilution

    Lane 1 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: NIH/3T3 (PMID:24324740).

  • Western blot - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    Western blot - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) at 1/1000 dilution + mouse testis lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling LINE-1 ORF1p with ab216324 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control: No staining on mouse liver (PMID: 24607009) is observed. Counterstained with hematoxylin.
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labeling LINE-1 ORF1p with ab216324 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining in F9 cell line.

    Negative control: NIH/3T3 (PMID: 24324740).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).

  • Flow Cytometry - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    Flow Cytometry - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast, Left) / F9 (mouse embryonal carcinoma epithelial cell, Right) cell lines labeling LINE-1 ORF1p with ab216324 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: NIH/3T3 (PMID:24324740).

  • Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)

    LINE-1 ORF1p was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate with ab216324 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216324 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution

    Lane 1: F9 whole cell lysate 10 µg (Input).
    Lane 2: ab216324 IP in F9 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216324 in F9 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
    Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)

© 2021 Astana Biomed Group LLP. All rights reserved.