Anti-Lgi1/EPT antibody (ab30868)
Key features and details
- Rabbit polyclonal to Lgi1/EPT
- Suitable for: IHC-FoFr, ICC/IF, WB
- Reacts with: Mouse, Rat, Hamster, Human
- Isotype: IgG
Overview
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Product name
Anti-Lgi1/EPT antibody
See all Lgi1/EPT primary antibodies -
Description
Rabbit polyclonal to Lgi1/EPT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-FoFr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Hamster, Human
Predicted to work with: Chicken, Cow
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-FoFr (1) Use at an assay dependent concentration.ICC/IF (1) Use at an assay dependent concentration.WB (3) Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).Notes IHC-FoFr
Use at an assay dependent concentration.ICC/IF
Use at an assay dependent concentration.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).Target
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Function
Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival. -
Tissue specificity
Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level). -
Involvement in disease
Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs. -
Sequence similarities
Contains 7 EAR repeats.
Contains 3 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 617080 Cow
- Entrez Gene: 9211 Human
- Entrez Gene: 56839 Mouse
- Entrez Gene: 252892 Rat
- Omim: 604619 Human
- SwissProt: O95970 Human
- SwissProt: Q9JIA1 Mouse
- SwissProt: Q8K4Y5 Rat
see all -
Alternative names
- ADLTE antibody
- ADPAEF antibody
- ADPEAF antibody
see all
Images
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Western blot - Anti-Lgi1/EPT antibody (ab30868)All lanes : Anti-Lgi1/EPT antibody (ab30868) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse brain P0 tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 64 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesGel type: MOPS
Blocking buffer: 2% BSA block
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Western blot - Anti-Lgi1/EPT antibody (ab30868)This image is courtesy of an anonymous AbreviewAnti-Lgi1/EPT antibody (ab30868) at 1/1000 dilution + rat brain tissue lysate at 30 µg
Secondary
HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 5 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab30868 has been referenced in 28 publications.
- Hu P et al. A novel LGI1 mutation causing autosomal dominant lateral temporal lobe epilepsy confirmed by a precise knock-in mouse model. CNS Neurosci Ther 28:237-246 (2022). PubMed: 34767694
- Liu F et al. A novel LGI1 missense mutation causes dysfunction in cortical neuronal migration and seizures. Brain Res 1721:146332 (2019). PubMed: 31301272
- Silva J et al. Selective inactivation of LGI1 in neuronal precursor cells leads to cortical dysplasia in mice. Genesis N/A:e23268 (2018). PubMed: 30489685
- Zhou L et al. Celecoxib Ameliorates Seizure Susceptibility in Autosomal Dominant Lateral Temporal Epilepsy. J Neurosci 38:3346-3357 (2018). PubMed: 29491011
- Jang Y et al. LGI1 expression and human brain asymmetry: insights from patients with LGI1-antibody encephalitis. J Neuroinflammation 15:279 (2018). PubMed: 30253786
Images
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Western blot - Anti-Lgi1/EPT antibody (ab30868)All lanes : Anti-Lgi1/EPT antibody (ab30868) at 1 µg/ml
Lane 1 : Mouse brain whole tissue lysate
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Rat brain whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR Dye 680 Conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 64 kDa -
Western blot - Anti-Lgi1/EPT antibody (ab30868) This image is courtesy of an anonymous AbreviewAnti-Lgi1/EPT antibody (ab30868) at 1/1000 dilution + rat brain tissue lysate at 30 µg
Secondary
HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 5 minutes
