Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Leukotriene B4 Receptor 2 antibody (ab84600)

Key features and details

  • Rabbit polyclonal to Leukotriene B4 Receptor 2
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Leukotriene B4 Receptor 2 antibody

  • Description

    Rabbit polyclonal to Leukotriene B4 Receptor 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to Human Leukotriene B4 Receptor 2 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab94553)

  • Positive control

    • This antibody gave a positive signal in Human brain tissue lysate.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab84600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

Images

  • Western blot - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)
    Western blot - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)
    Anti-Leukotriene B4 Receptor 2 antibody (ab84600) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 15 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)
    Immunocytochemistry/ Immunofluorescence - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)
    ICC/IF image of ab84600 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84600, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References (0)

Publishing research using ab84600? Please let us know so that we can cite the reference in this datasheet.

ab84600 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)
    Western blot - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)
    Anti-Leukotriene B4 Receptor 2 antibody (ab84600) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 15 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)
    Immunocytochemistry/ Immunofluorescence - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)
    ICC/IF image of ab84600 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84600, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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