Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
![Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)](https://www.abcam.com/ps/products/109/ab109434/Images/ab109434-395531-anti-leukotriene-a4-hydrolaselta4h-antibody-epr5712-western-blot-human-knockout-lysate.jpg "Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5712] to Leukotriene A4 hydrolase/LTA4H
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712]
See all Leukotriene A4 hydrolase/LTA4H primary antibodies -
Description
Rabbit monoclonal [EPR5712] to Leukotriene A4 hydrolase/LTA4H -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, 293T, T47-D, and A549 cell lysates. IHC-P: Human breast carcinoma tissue.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as Leukotriene A4 hydrolase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5712 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : LTA4H knockout HEK293T cell lysate
Lane 3 : T-47D cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDaLanes 1-4: Merged signal (red and green). Green - ab109434 observed at 69 kDa. Red - loading control ab8245 observed at 36 kDa.
ab109434 Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] was shown to specifically react with Leukotriene in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266467 (knockout cell lysate ab258034) was used. Wild-type and Leukotriene knockout samples were subjected to SDS-PAGE. ab109434 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)](https://www.abcam.com/ps/products/109/ab109434/Images/ab109434-122272-anti-leukotriene-a4-hydrolase-antibody-epr5712-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)Immunohistochemical analysis of Leukotriene A4 hydrolase/LTA4H in paraffin-embedded Human breast carcinoma tissue using ab109434 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)](https://www.abcam.com/ps/products/109/ab109434/Images/ab109434-122273-anti-leukotriene-a4-hydrolase-antibody-epr5712-western-blot.jpg)
Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : T47D cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 69 kDa
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![OI-RD Scanning - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)](https://www.abcam.com/ps/products/109/ab109434/Images/ab109434-122271-anti-leukotriene-a4-hydrolase-antibody-epr5712.jpg)
OI-RD Scanning - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434) -
![Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)](https://www.abcam.com/ps/products/109/ab109434/Images/ab109434-5-benefits-of-recombinant-antibodies.png)
Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
