Anti-Leukotriene A4 hydrolase/LTA4H antibody (ab240741)
Key features and details
- Rabbit polyclonal to Leukotriene A4 hydrolase/LTA4H
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Leukotriene A4 hydrolase/LTA4H antibody
See all Leukotriene A4 hydrolase/LTA4H primary antibodies -
Description
Rabbit polyclonal to Leukotriene A4 hydrolase/LTA4H -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide within Human Leukotriene A4 hydrolase/LTA4H aa 561-611. The exact sequence is proprietary.
Database link: P09960 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
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General notes
This product was previously labelled as Leukotriene A4 hydrolase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Leukotriene A4 hydrolase/LTA4H immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody (ab240741) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 3 minutes
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Leukotriene A4 hydrolase/LTA4H was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded prepared using NETN lysis buffer) using ab240741 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab240741 at 1 μg/mL.
Lane 1: ab240741 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 3 minutes.