Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21199] to Laminin gamma 1
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Laminin gamma 1 antibody [EPR21199]
See all Laminin gamma 1 primary antibodies
Description

Rabbit monoclonal [EPR21199] to Laminin gamma 1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A431, NIH:OVCAR-3, HeLa, MCF7, C6, PC-12 and A549 whole cell lysates; Human fetal heart and fetal kidney tissue lysates; rat kidney, brain and spleen tissue lysates; mouse brain tissue lysate. IP: A431 whole cell lysate. IHC-P: Human pancreas tissue; mouse kidney tissue; rat stomach tissue. Flow cyt: A431 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21199
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in basement membrane of human pancreas (PMID: 15159456) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A431 (human epidermoid carcinoma cell line) cell line labeling Laminin gamma 1 with ab233389 at 1/50 (red) compared with Rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Laminin gamma 1 was immunoprecipitated from 0.35 mg of A431 (human epidermoid carcinoma cell line) whole cell lysate with ab233389 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233389 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: A431 whole lysate 10 μg (Input).
Lane 2: ab233389 IP in A431 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233389 in A431 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in basement membrane of rat stomach (PMID: 12571278) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in the basement membrane of mouse kidney (PMID: 21903675) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

All lanes : Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/5000 dilution

Lane 1 : C6 (rat glial tumor glial cell line) whole cell lysate at 10 µg
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 3 : Rat heart lysate at 10 µg
Lane 4 : Rat kidney lysate at 10 µg
Lane 5 : Rat brain lysate at 10 µg
Lane 6 : Rat spleen lysate at 10 µg
Lane 7 : Mouse brain lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 178 kDa
Observed band size: 210-250 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 70 seconds. Lane 2: 15 seconds. Lanes 3 & 4: 10 seconds. Lanes 5 & 6: 3 minutes. Lane 7: 48 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 18263645, 11067943, 23857767, 28928891).
Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Lanes 1-3 & 6-7 : Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/5000 dilution
Lanes 4-5 : Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/1000 dilution

Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : NIH:OVCAR-3 (human ovary adenocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 6 : Human fetal heart lysate
Lane 7 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 6-7 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 178 kDa
Observed band size: 210-250 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1, 2 & 7: 15 seconds. Lane 3: 8 seconds. Lanes 4: 37 seconds. Lanes 5 & 6: 10 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 18263645, 11067943, 23857767, 28928891).
Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

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