Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21199] to Laminin gamma 1
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Laminin gamma 1 antibody [EPR21199]
    See all Laminin gamma 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21199] to Laminin gamma 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, NIH:OVCAR-3, HeLa, MCF7, C6, PC-12 and A549 whole cell lysates; Human fetal heart and fetal kidney tissue lysates; rat kidney, brain and spleen tissue lysates; mouse brain tissue lysate. IP: A431 whole cell lysate. IHC-P: Human pancreas tissue; mouse kidney tissue; rat stomach tissue. Flow cyt: A431 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in basement membrane of human pancreas (PMID: 15159456) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    Flow Cytometry - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A431 (human epidermoid carcinoma cell line) cell line labeling Laminin gamma 1 with ab233389 at 1/50 (red) compared with Rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    Immunoprecipitation - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

    Laminin gamma 1 was immunoprecipitated from 0.35 mg of A431 (human epidermoid carcinoma cell line) whole cell lysate with ab233389 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233389 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: A431 whole lysate 10 μg (Input).
    Lane 2: ab233389 IP in A431 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233389 in A431 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 15 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in basement membrane of rat stomach (PMID: 12571278) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in the basement membrane of mouse kidney (PMID: 21903675) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    All lanes : Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/5000 dilution

    Lane 1 : C6 (rat glial tumor glial cell line) whole cell lysate at 10 µg
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
    Lane 3 : Rat heart lysate at 10 µg
    Lane 4 : Rat kidney lysate at 10 µg
    Lane 5 : Rat brain lysate at 10 µg
    Lane 6 : Rat spleen lysate at 10 µg
    Lane 7 : Mouse brain lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 178 kDa
    Observed band size: 210-250 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 70 seconds. Lane 2: 15 seconds. Lanes 3 & 4: 10 seconds. Lanes 5 & 6: 3 minutes. Lane 7: 48 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 18263645, 11067943, 23857767, 28928891).

  • Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    Lanes 1-3 & 6-7 : Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/5000 dilution
    Lanes 4-5 : Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : NIH:OVCAR-3 (human ovary adenocarcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 6 : Human fetal heart lysate
    Lane 7 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 6-7 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 178 kDa
    Observed band size: 210-250 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1, 2 & 7: 15 seconds. Lane 3: 8 seconds. Lanes 4: 37 seconds. Lanes 5 & 6: 10 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 18263645, 11067943, 23857767, 28928891).

  • Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
    Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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