Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5367-62] to Lambda Light chain
  • Suitable for: Flow Cyt, WB, ELISA, IHC-P, ICC/IF
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Lambda Light chain antibody [EPR5367-62]
    See all Lambda Light chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5367-62] to Lambda Light chain

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Human Lambda Light chain. Purified Human IgA.

  • Positive control

    • WB: Human tonsil, plasma, thymus and spleen lysates. IHC-P: Human tonsil and colon tissues. ICC/IF: Ramos cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5367-62

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Flow Cytometry - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling Lambda Light chain with unpurified ab124719 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/100000 dilution (purified) + Human plasma lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/20000 dilution (purified) + Human thymus tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lambda Light chain with purified ab124719 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling Lambda Light chain with purified ab124719 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    ELISA analysis of Human Kappa light chain (Free), Human Lambda Light Chains (Free), Human IgA, Human IgM, Human IgG, Rat IgG, Mouse IgG at 1000 ng/mL with ab124719 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    All lanes : Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/50000 dilution (unpurified)

    Lane 1 : Human tonsil lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Lambda Light chain with unpurified ab124719 at a dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
    Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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