Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5367-62] to Lambda Light chain
Suitable for: Flow Cyt, WB, ELISA, IHC-P, ICC/IF
Reacts with: Human, Recombinant fragment

Product name

Anti-Lambda Light chain antibody [EPR5367-62]
See all Lambda Light chain primary antibodies
Description

Rabbit monoclonal [EPR5367-62] to Lambda Light chain
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Full length native protein (purified) corresponding to Human Lambda Light chain. Purified Human IgA.
Positive control
- WB: Human tonsil, plasma, thymus and spleen lysates. IHC-P: Human tonsil and colon tissues. ICC/IF: Ramos cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5367-62
Isotype

IgG
Research areas

Flow Cytometry - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling Lambda Light chain with unpurified ab124719 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/100000 dilution (purified) + Human plasma lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/20000 dilution (purified) + Human thymus tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lambda Light chain with purified ab124719 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling Lambda Light chain with purified ab124719 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

ELISA analysis of Human Kappa light chain (Free), Human Lambda Light Chains (Free), Human IgA, Human IgM, Human IgG, Rat IgG, Mouse IgG at 1000 ng/mL with ab124719 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

All lanes : Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/50000 dilution (unpurified)

Lane 1 : Human tonsil lysate
Lane 2 : Human plasma lysate
Lane 3 : Human spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Lambda Light chain with unpurified ab124719 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

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