Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8266] to LAMA3 - BSA and Azide free
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-LAMA3 antibody [EPR8266] - BSA and Azide free
    See all LAMA3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8266] to LAMA3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, SH-SY5Y and A431 cell lysates; Human skin tissue lysate. ICC: HepG2 cells.

  • General notes

    ab249033 is the carrier-free version of ab151715 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249033 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Laminin subunit alpha-3, Laminin 5 alpha 3

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)
    Western blot - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)
    All lanes : Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : LAMA3 knockout HeLa cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 367 kDa
    Observed band size: 367 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab151715).

    Lanes 1 - 3: Merged signal (red and green). Green - ab151715 observed at 367 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab151715 was shown to react with LAMA3 in wild-type HeLa cells in western blot with loss of signal observed in LAMA3 knockout cell line ab265663 (LAMA3 knockout cell lysate ab257497). Wild-type and LAMA3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab151715 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)
    Immunocytochemistry - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)

    This data was developed using ab151715, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of HepG2 cells labeling LAMA3 with ab151715 at 1/100 dilution.

  • Western blot - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)
    Western blot - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)
    All lanes : Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human skin lysate
    Lane 5 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 367 kDa



    This data was developed using ab151715, the same antibody clone in a different buffer formulation.

     

  • Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)
    Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)

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