Anti-L-Kynurenine Hydrolase antibody (ab225916)
Key features and details
- Rabbit polyclonal to L-Kynurenine Hydrolase
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-L-Kynurenine Hydrolase antibody
See all L-Kynurenine Hydrolase primary antibodies -
Description
Rabbit polyclonal to L-Kynurenine Hydrolase -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human L-Kynurenine Hydrolase aa 1-307.
Database link: Q16719 -
Positive control
- IHC-P: Human liver and placenta tissues. WB: A549, HeLa and HepG2 whole cell lysates. IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-L-Kynurenine Hydrolase antibody (ab225916)All lanes : Anti-L-Kynurenine Hydrolase antibody (ab225916) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 35,52 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L-Kynurenine Hydrolase antibody (ab225916)Paraffin-embedded human liver tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L-Kynurenine Hydrolase antibody (ab225916)Paraffin-embedded human placenta tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-L-Kynurenine Hydrolase antibody (ab225916)L-Kynurenine Hydrolase was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg ab225916. For Western Blot analysis, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.
Lane 2: ab225916 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
