Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (ab250831)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15452(B)] to Methionyl Aminopeptidase 1/MAP 1 - BSA and Azide free
- Suitable for: IHC-P, ICC, Flow Cyt, WB
- Reacts with: Rat, Human
Overview
-
Product name
Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free
See all Methionyl Aminopeptidase 1/MAP 1 primary antibodies -
Description
Rabbit monoclonal [EPR15452(B)] to Methionyl Aminopeptidase 1/MAP 1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab250831 is the carrier-free version of ab185540 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250831 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Methionyl Aminopeptidase 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15452(B) -
Isotype
IgG -
Research areas
Images
-
This data was developed using ab185540, the same antibody clone in a different buffer formulation.
Flow cytometry analysis of 2% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/180 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. -
This data was developed using ab185540, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Counterstained with Dapi blue.
-
This data was developed using ab185540, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.
-
This data was developed using ab185540, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
This data was developed using ab185540, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
All lanes : Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDaThis data was developed using ab185540, the same antibody clone in a different buffer formulation.
-