Anti-Ku80 antibody (ab236277)
Key features and details
- Rabbit polyclonal to Ku80
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Ku80 antibody
See all Ku80 primary antibodies -
Description
Rabbit polyclonal to Ku80 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Ku80 aa 250-500.
Database link: P13010 -
Positive control
- WB: HepG2, L02, A549 and MCF7 whole cell lysates; Mouse liver lysate. IP: HepG2 whole cell lysate. IHC-P: Human melanoma, colon cancer, brain and tonsil tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Ku80 antibody (ab236277) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : L02 (human immortal liver cell line) whole cell lysate
Lane 3 : Mouse liver lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 83 kDa
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All lanes : Anti-Ku80 antibody (ab236277) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 83 kDa
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Ku 80 was immunoprecipitated from 0.5 mg HepG2 human (liver hepatocellular carcinoma cell line) whole cell lysate with ab236277 at 1/200 dilution. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 1: Control rabbit IgG IP (1 μg) in HepG2 whole cell lysate.
Lane 2: ab236277 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 μg (Input).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody (ab236277)
Paraffin-embedded human melanoma tissue stained for Ku80 using ab236277 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody (ab236277)
Paraffin-embedded human colon cancer tissue stained for Ku80 using ab236277 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody (ab236277)
Paraffin-embedded human brain tissue stained for Ku80 using ab236277 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody (ab236277)
Paraffin-embedded human tonsil tissue stained for Ku80 using ab236277 at 1/100 dilution in immunohistochemical analysis.