Anti-KMT1A / SUV39H1 antibody [3HCLC] (ab277804)
![Anti-KMT1A / SUV39H1 antibody [3HCLC] (ab277804)](https://www.abcam.com/ps/products/277/ab277804/Images/ab277804-1-antiSUV39H1recombinantpolyclonalantibody3hclcWB.jpg "Anti-KMT1A / SUV39H1 antibody [3HCLC] (ab277804)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [3HCLC] to KMT1A / SUV39H1
- Suitable for: WB
- Reacts with: Human, African green monkey
Overview
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Product name
Anti-KMT1A / SUV39H1 antibody [3HCLC]
See all KMT1A / SUV39H1 primary antibodies -
Description
Rabbit recombinant multiclonal [3HCLC] to KMT1A / SUV39H1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human KMT1A/ SUV39H1 aa 65-214.
Database link: O43463 -
Positive control
- WB: HCT116, COS7, A431, HeLa, HepG2 and A549 nuclear extracts.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
3HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KMT1A / SUV39H1 antibody (ab277804)
Western blot analysis was performed on nuclear extracts (30 μg lysate) of HCT 116 (human colorectal carcinoma cell line) (Lane 1), COS7 (African green monkey kidney fibroblast-like cell line) (Lane 2), A431 (human epidermoid carcinoma cell line) (Lane 3), HeLa (human epithelial cell line from cervix adenocarcinoma) (Lane 4), HepG2 (human liver hepatocellular carcinoma cell line) (Lane 5) and A549 (human lung carcinoma cell line) (Lane 6).
The blots were probed with ab277804, 1-2 μg/mL and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L)-HRP secondary antibody, (0.4 μg/mL, 1/2500 dilution).
4-12% Bis-Tris gel.
The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed.
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![Anti-KMT1A / SUV39H1 antibody [3HCLC] (ab277804)](https://www.abcam.com/ps/products/277/ab277804/Images/ab277804-455724-benefits-of-recombinant-antibodies.jpg)
Anti-KMT1A / SUV39H1 antibody [3HCLC] (ab277804)