Anti-wide spectrum Cytokeratin antibody (ab9377)
Key features and details
- Rabbit polyclonal to wide spectrum Cytokeratin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-wide spectrum Cytokeratin antibody -
Description
Rabbit polyclonal to wide spectrum Cytokeratin -
Host species
Rabbit -
Specificity
This antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
Cytokeratin isolated from bovine muzzle epidermis.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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ab9377 staining wide spectrum Cytokeratin in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry.
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ab17976 staining wide spectrum Cytokeratin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with BlockAid for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/500 in 1% BSA + PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.
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ab9377 staining wide spectrum Cytokeratin in rat liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
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ab9377 staining wide spectrum Cytokeratin in common marmoset lung tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
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Ab9377 positively staining Cytokeratin (green) in paraffin fixed mouse oesophagus at 1/250.