Anti-KLF5 antibody [OTI2G4] - BSA and Azide free (ab273672)
![Anti-KLF5 antibody [OTI2G4] - BSA and Azide free (ab273672)](https://www.abcam.com/ps/products/273/ab273672/Images/ab273672-394427-AntiKLF5-antibody-OTI2G4-immunohistochemistry-placenta-tissue-human.JPG "Anti-KLF5 antibody [OTI2G4] - BSA and Azide free (ab273672)")
Key features and details
- Mouse monoclonal [OTI2G4] to KLF5 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-KLF5 antibody [OTI2G4] - BSA and Azide free
See all KLF5 primary antibodies -
Description
Mouse monoclonal [OTI2G4] to KLF5 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human KLF5 aa 1 to the C-terminus. NP_001721
Database link: Q13887 -
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY KLF5 cDNA cell lysate. HT-29, A549, HeLa and U-87 MG cell extracts. IHC-P: Human placenta tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OTI2G4 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody [OTI2G4] - BSA and Azide free (ab273672)
Paraffin embedded human placenta tissue stained for KLF5 using the same antibody clone in a different buffer formulation (PBS, BSA, glycerol, and sodium azide) at 1/500 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3mins.
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![Western blot - Anti-KLF5 antibody [OTI2G4] - BSA and Azide free (ab273672)](https://www.abcam.com/ps/products/273/ab273672/Images/ab273672-394426-AntiKLF5antibodyOTI2G4westernblotHT29A549HeLaandU87human.jpg)
Western blot - Anti-KLF5 antibody [OTI2G4] - BSA and Azide free (ab273672)All lanes : Same antibody clone in a different buffer formulation at 1/500 dilution
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
Lane 2 : A549 (Human lung carcinoma cell line) cell extract
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 51 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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![Western blot - Anti-KLF5 antibody [OTI2G4] - BSA and Azide free (ab273672)](https://www.abcam.com/ps/products/273/ab273672/Images/ab273672-394425-AntiKLF5antibodyOTI2G4westernblottransfectedHEK293T.jpg)
Western blot - Anti-KLF5 antibody [OTI2G4] - BSA and Azide free (ab273672)All lanes : Same antibody clone in a different buffer formulation at 1/2000 dilution
Lane 1 : HEK-293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY KLF5 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 51 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.