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Epigenetics and Nuclear Signaling Chromosome Structure Chromosome

Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)

Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5600] to KIFAP3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free
    See all KIFAP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5600] to KIFAP3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248540 is the carrier-free version of ab133537.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.05 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5600
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Chromosome
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Western blot - Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)
    Western blot - Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)
    All lanes : Anti-KIFAP3 antibody [EPR5600] (ab133537) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 91 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133537, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)
    This data was developed using ab133537, the same antibody clone in a different buffer formulation.Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling KIFAP3 with ab133537 at 1/50 Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)
    OI-RD Scanning - Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)
    This data was developed using ab133537, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)
    Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (ab248540)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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