Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-KIF5C antibody [EPR16224-32] (ab193352)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16224-32] to KIF5C
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-KIF5C antibody [EPR16224-32]
See all KIF5C primary antibodies
Description

Rabbit monoclonal [EPR16224-32] to KIF5C
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Neuro-2a, Human fetal brain, SH-SY5Y, mouse brain, rat brain and C6 lysate. Human testis tissue. Mouse cerebral cortex tissue. SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16224-32
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-KIF5C antibody [EPR16224-32] (ab193352)

Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling KIF5C using ab193352 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.
Western blot - Anti-KIF5C antibody [EPR16224-32] (ab193352)

All lanes : Anti-KIF5C antibody [EPR16224-32] (ab193352) at 1/10000 dilution

Lane 1 : Neuro-2a lysate
Lane 2 : Human fetal brain lysate
Lane 3 : SH-SY5Y lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 109 kDa
Western blot - Anti-KIF5C antibody [EPR16224-32] (ab193352)

All lanes : Anti-KIF5C antibody [EPR16224-32] (ab193352) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 109 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] (ab193352)

Immunohistochemical analysis of paraffin embedded Human testis tissue sections labeling KIF5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] (ab193352)

Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue sections labeling KIF5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-KIF5C antibody [EPR16224-32] (ab193352)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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