Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIF5C antibody [EPR16224-32] (ab193352)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16224-32] to KIF5C
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KIF5C antibody [EPR16224-32]
    See all KIF5C primary antibodies

  • Description

    Rabbit monoclonal [EPR16224-32] to KIF5C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Neuro-2a, Human fetal brain, SH-SY5Y, mouse brain, rat brain and C6 lysate. Human testis tissue. Mouse cerebral cortex tissue. SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16224-32

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-KIF5C antibody [EPR16224-32] (ab193352)
    Immunocytochemistry/ Immunofluorescence - Anti-KIF5C antibody [EPR16224-32] (ab193352)

    Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling KIF5C using ab193352 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

  • Western blot - Anti-KIF5C antibody [EPR16224-32] (ab193352)
    Western blot - Anti-KIF5C antibody [EPR16224-32] (ab193352)
    All lanes : Anti-KIF5C antibody [EPR16224-32] (ab193352) at 1/10000 dilution

    Lane 1 : Neuro-2a lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : SH-SY5Y lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa

  • Western blot - Anti-KIF5C antibody [EPR16224-32] (ab193352)
    Western blot - Anti-KIF5C antibody [EPR16224-32] (ab193352)
    All lanes : Anti-KIF5C antibody [EPR16224-32] (ab193352) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] (ab193352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] (ab193352)

    Immunohistochemical analysis of paraffin embedded Human testis tissue sections labeling KIF5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] (ab193352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] (ab193352)

    Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue sections labeling KIF5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-KIF5C antibody [EPR16224-32] (ab193352)
    Anti-KIF5C antibody [EPR16224-32] (ab193352)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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