Anti-KIF4A/KIF4 antibody (ab72412)
Key features and details
- Rabbit polyclonal to KIF4A/KIF4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIF4A/KIF4 antibody
See all KIF4A/KIF4 primary antibodies -
Description
Rabbit polyclonal to KIF4A/KIF4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human KIF4A/KIF4 (C terminal).
Database link: NP_036442.3 -
Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab72412 was affinity purified using an epitope specific to KIF4A/KIF4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KIF4A/KIF4 antibody (ab72412)All lanes : Anti-KIF4A/KIF4 antibody (ab72412) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 140 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunoprecipitation - Anti-KIF4A/KIF4 antibody (ab72412)Detection of KIF4A/KIF4 by Western Blot of Immunprecipitate.
ab72412 at 1µg/ml staining KIF4A/KIF4 in HeLa whole cell lysate immunoprecipitated using ab72412 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Detection: Chemiluminescence with an exposure time of 10 seconds.
