Anti-Kif2a antibody (ab71160)
Key features and details
- Rabbit polyclonal to Kif2a
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Kif2a antibody
See all Kif2a primary antibodies -
Description
Rabbit polyclonal to Kif2a -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 630 and 679 of human Kif2a (NP_004511.1)
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Positive control
- Whole cell lysates from Hela cells, 293T cells and NIH3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Kif2a antibody (ab71160)All lanes : Anti-Kif2a antibody (ab71160) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-Kif2a antibody (ab71160)Detection of Human Kif2a by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab71160 at 3 µg/mg lysate for IP (Lane 1) and at 0.1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
