Anti-KIAA0528 antibody (ab80470)
Key features and details
- Rabbit polyclonal to KIAA0528
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KIAA0528 antibody -
Description
Rabbit polyclonal to KIAA0528 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Sea urchin, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to a region between residue 375 and 425 of human KIAA0528 ( NP_055617.1)
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0528 antibody (ab80470)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labelling KIAA0528 with ab80470 at 1/400 (2.5µg/ml). Detection: DAB.
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Western blot - Anti-KIAA0528 antibody (ab80470)All lanes : Anti-KIAA0528 antibody (ab80470) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 110 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
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Immunoprecipitation - Anti-KIAA0528 antibody (ab80470)Lane 1: ab80470, at 1 µg/mg, staining KIAA0528 in HeLa whole cell lysate by Western blot of immunoprecipitate (1 mg of lysate for IP, 20% of IP loaded). Immunoprecipitation was performed by using ab80470 at 3 µg/mg of lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.