Anti-KIAA0020 antibody (ab156692)
Key features and details
- Rabbit polyclonal to KIAA0020
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIAA0020 antibody
See all KIAA0020 primary antibodies -
Description
Rabbit polyclonal to KIAA0020 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region within amino acids 1-50 of Human KIAA0020 (NP_055693.4).
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab156692 was affinity purified using an epitope specific to KIAA0020 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KIAA0020 antibody (ab156692)All lanes : Anti-KIAA0020 antibody (ab156692) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-KIAA0020 antibody (ab156692)Detection of KIAA0020 by Western blot of immunoprecipitate.
Immunoprecipitation of KIAA0020 (6 µg of antibody per mg of HeLa whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: ab156692
Lane 2: alternative anti-KIAA0020 antibody
Lane 3: Control IgG
For the subsequent Western blot of immunoprecipitates, ab156692 was used at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lane 1 : Anti-KIAA0020 antibody (ab156692) at 1 µg/ml
Lane 2 : Alternative rabbit anti-KIAA0020 antibody
Lane 3 : Control IgG
All lanes : HeLa whole cell lysate
