Anti-Filamin A antibody (ab264184)
Key features and details
- Rabbit polyclonal to Filamin A
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Filamin A antibody
See all Filamin A primary antibodies -
Description
Rabbit polyclonal to Filamin A -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Filamin A aa 1725-1775. The exact sequence is proprietary.
Database link: P21333 -
Positive control
- IHC-P: Human colon carcinoma tissue. WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Filamin A antibody (ab264184)All lanes : Anti-Filamin A antibody (ab264184) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 1 second4 - 8% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody (ab264184)Immunohistochemical analysis of formalin/PFA-fixed, paraffin-embedded human colon carcinoma tissue labeling Filamin A with ab264184 at 1/1000 dilution. DAB was used as a counterstain.
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Immunoprecipitation - Anti-Filamin A antibody (ab264184)Filamin A was immunoprecipitated from 1mg of HeLa cells (20% of IP loaded) using ab264184 at 3 µg/mg lysate. Western blot was performed on the immunprecipitate using ab264184 at 0.1μg/ml.
Lane 1: ab264184 in HeLa whole cell lysate
Lane 2: Control IgG in HeLa whole cell lysate
Detected with chemilimuniscence with exposure time of 1 second.
